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. 2002 Jan 21;156(2):271–286. doi: 10.1083/jcb.200109077

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Gradient fractionation of vps-ts sec6-4 mutants. Strains were grown at 24°C in SD with required amino acids for maintaining plasmids (vps4-ts sec6, EHY348; pep12-ts sec6, EHY413) or in YPD (vps27-ts sec6, EHY374; vps4Δ sec6, EHY327). Strains grown in SD were shifted to YPD at 24°C for 2 h before 30- or 60-min shifts to 37°C in prewarmed YPD, pH 4.5. (Bottom right) A single culture of vps4Δ sec6 cells was split in half; one half was maintained at 24°C and the other half shifted to 32°C (semi-permissive for sec6-4) for 1h. Cells were fractionated as for Fig. 1, and gradient fractions were assayed for invertase and exoglucanase activities.