Table I. S. cerevisiae strains used in this study.
| Strain | Relevant genotypea | Source |
|---|---|---|
| DBY1829 | MATα his3-Δ200 leu2-3,112 lys2-801 trp1-1 ura3-52 | T. Huffakerb |
| NY10 | MATα ura3-52 | P. Novickc |
| NY17 | MATa sec6-4 ura3-52 | Salminen and Novick, 1987 |
| CBY31 | MATα pep12Δ::HIS3 his3-Δ200 leu2-3,112 lys2-801 trp1-Δ901 ura3-52 suc2-Δ9 | Burd et al., 1997 |
| CCY255 | MATα apl6Δ::HIS3 his3-Δ200 leu2-3,112 lys2-801 trp1-Δ901 ura3-52 suc2-Δ9 | Cowles et al., 1997a |
| WSY41 | MATα vps41Δ::LEU2 his3-Δ200 leu2-3,112 lys2-801 trp1-Δ901 ura3-52 suc2-Δ9 | Cowles et al., 1997b |
| MBY3 | MATα vps4Δ::TRP1 his3-Δ200 leu2-3,112 lys2-801 trp1-Δ901 ura3-52 suc2-Δ9 | Babst et al., 1997 |
| RPY2 | MATa vps27-123(ts) leu2-3,112 ura3-52 his4-519 ade6 | Piper et al., 1995 |
| TDY2 | MATα vam3Δ::LEU2 his3-Δ200 leu2-3,112 lys2-801 trp1-Δ901 ura3-52 suc2-Δ9 [vam3-ts URA3] | Darsow et al., 1997 |
| EHY47 | MATa his3-Δ200 leu2-3,112 trp1-1 ura3-52 | NY17 × DBY1829 |
| EHY50 | MATa sec6-4 his3-Δ200 leu2-3,112 trp1-1 ura3-52 | NY17 × DBY1829 |
| EHY52 | MATα sec6-4 his3-Δ200 leu2-3,112 lys2-801 trp1-1 ura3-52 | NY17 × DBY1829 |
| EHY62 | MATα sec6-4 vps10Δ::TRP1 his3-Δ200 leu2-3,112 lys2-801 trp1-1 ura3-52 | EHY52, integ. pEMY10-3 |
| EHY188 | MATα sec6-4 TPI::SUC2::HIS3 his3-Δ200 leu2-3,112 lys2-801 trp1-1 ura3-52 | EHY52, integ. pEH117 |
| EHY191 | MATa ade2-1 his3-11,15 leu2-3,112 trp1-Δ ura3-1 pep4Δ::TRP1 TPI::SUC2::HIS3 | RSY 620, integ. pEH117 |
| EHY225 | MATα sec6-4 vps1::LEU2 TPI::SUC2::HIS3 his3-Δ200 leu2-3,112 lys2-801 trp1-1 ura3-5 | EHY188, integ. pCKR2 |
| EHY226 | MATa sec6-4 TPI::SUC2::URA3 his3-Δ200 leu2-3,112 trp1-1 ura3-52 | EHY50, integ. pEH119 |
| EHY227 | MATa sec6-4 TPI::SUC2::TRP1 his3-Δ200 leu2-3,112 trp1-1 ura3-52 | EHY50, integ. pEH118 |
| EHY232 | MATa sec6-4 pep12Δ::HIS3 TPI::SUC2::TRP1 his3-Δ200 leu2-3,112 trp1 ura3-52 | EHY227 × CBY31 |
| EHY239 | MATa TPI::SUC2::TRP1 his3-Δ200 leu2-3,112 trp1-1 ura3-52 | EHY47, integ. pEH118 |
| EHY242 | MATα sec6-4 chc1-521(ts) TPI::SUC2::URA3 his3-Δ200 ura3-5 | Materials and methods |
| EHY282 | MATα sec6-4 vps10Δ::TRP1 TPI::SUC2::URA3 his3-Δ200 leu2-3,112 lys2-801 trp1-1 ura3-52 | EHY62, integ. pEH119 |
| EHY309 | MATa sec6-4 vps27Δ::LEU2 TPI::SUC2::TRP1 his3-Δ200 leu2-3,112 trp1-1 ura3-52 | EHY227, integ. pKJH2 |
| EHY326 | MATα sec6-4 vps41Δ::LEU2 TPI::SUC2::URA3 his3-Δ200 leu2-3,112 trp1 ura3-52 | EHY226 × WSY41 |
| EHY327 | MATα sec6-4 vps4Δ::TRP1 TPI::SUC2::URA3 his3-Δ200 leu2-3,112 trp1 ura3-52 | EHY226 × MBY3 |
| EHY348 | MATα sec6-4 vps4Δ::TRP1 TPI::SUC2::URA3 his3-Δ200 leu2-3,112 trp1 ura3-52 [vps4-ts HIS3] | EHY327, pMB59 |
| EHY351 | MATα sec6-4 apl6Δ::HIS3 TPI::SUC2::TRP1 his3-Δ200 leu2-3,112 trp1-Δ901 ura3-52 | EHY227 × CCY255 |
| EHY374 | MATa sec6-4 vps27-123(ts) TPI::SUC2::TRP1 leu2-3,112 ura3-52d | EHY227 × RPY2 |
| EHY376 | MATa TPI::SUC2::TRP1 his3-Δ200 leu2-3,112 trp1-1 ura3-52 [HA::KEX2 LEU2] | EHY227 × RPY2 |
| EHY413 | MATa sec6-4 pep12Δ::HIS3 TPI::SUC2::URA3 his3-Δ200 leu2-3,112 trp1 ura3-52 [pep12-ts TRP1] | EHY226 × CBY31, pCB49 |
| EHY432 | MATa sec6-4 TPI::SUC2::TRP1 his3-Δ200 leu2-3,112 trp1-1 ura3-52 [HA::KEX2 URA3] | EHY227, pSN218 |
| EHY436 | MATa sec6-4 vam3Δ::LEU2 TPI::SUC2::TRP1 his3-Δ200 leu2-3,112 trp1-Δ901 ura3-52 [vam3-ts URA3] | EHY227 × TDY2 |
| EHY350 | MATα sec6-4 vps41Δ::LEU2 TPI::SUC2::URA3 his3-Δ200 leu2-3,112 trp1 ura3-52 [vps41-ts TRP1] | EHY326, integ. pVPS41-85 |
| EHY478 | MATα sec6-4 vps4Δ::TRP1 TPI::SUC2::URA3 his3-Δ200 leu2-3,112 trp1 ura3-52 PMA1-HA::LEU2 | EHY327, integ. HA-PMA1 |
a
Genes born by plasmids are indicated by brackets.
b
Cornell University, Ithaca, NY.
c
Yale University, New Haven, CT.
d
May also be his4-519 and/or his3-Δ200.