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. 2002 Apr 15;157(2):327–336. doi: 10.1083/jcb.200110138

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Expression of PLP in BHK cells affects the sorting of GPI–YFP. (a) BHK cells were transfected with GPI–YFP and, after 24 h, were fixed and analyzed by immunofluorescence. (b) Cells were transfected with GPI–YFP, incubated for 24 h in the presence of 10 μg/ml U18666A, fixed, and analyzed by immunofluorescence. (c) BHK cells were cotransfected with PLP and GPI–YFP and analyzed after 24 h by immunofluorescence for PLP (red) and GPI–YFP (green). In the merged image, yellow indicates colocalization of PLP and GPI–YFP. Bars, 10 μm.