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. 2002 Apr 15;157(2):327–336. doi: 10.1083/jcb.200110138

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Copyright © 2002, The Rockefeller University Press

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Figure 7. — Exogenously expressed PLP is not transported to the processes but accumulates cholesterol in primary cultures of oligodendrocytes. (a) Oligodendrocytes were infected with SFV containing myc-tagged PLP and incubated with rhodamine–dextran for 2 h at 37°C during the last 2 h of infection. 8 h after infection, cells were fixed and processed for immunofluorescence. Exogenous PLP (green) is mainly found in the cell body. The panels at the right show the cell body at lower exposure. Colocalization of PLP (green) and rhodamine–dextran (red) is shown in the merged image as yellow. (b) Oligodendrocytes were infected with SFV containing myc-tagged PLP, and 8 h after infection, cells were fixed and processed for immunofluorescence for PLP (green) and calnexin (red). (c) Oligodendrocytes were infected with SFV containing myc-tagged PLP, and 16 h after infection, cells were fixed and processed for immunofluorescence for PLP (red) and cholesterol (blue). The region indicated in the upper panel is shown below at a higher magnification. Colocalization of PLP and cholesterol is shown in the merged image as pink. (d) Oligodendrocytes were infected with SFV containing MOG, and 16 h after infection, cells were fixed and processed for immunofluorescence for MOG (red) and cholesterol (blue). Bars, 10 μm.