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. 2002 Apr 15;157(2):315–326. doi: 10.1083/jcb.200110027

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — NPFX_(1,2)D-mediated endocytosis requires SLA1. (A and B) Centromere plasmids encoding Ste2p-G392N, Ste2p-NPFXD (Ste2p11KR-G392N), and Ste2p-GPFXD (Ste2p11KR) were introduced into SLA1 cells (GPY2490, GPY2454, and GPY2665), and the same plasmids plus Ste2p-Ub (Ste2p-G392A) were introduced into sla1Δ cells (GPY2487, GPY 2488, GPY2489, GPY2451, and GPY2666). Endocytosis was monitored by α-factor uptake as described in the legend to Fig. 1.