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. 2007 Oct 5;116(1):1–6. doi: 10.1289/ehp.10403

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PI-3K activation induced by arsenite in both HaCat and NHEKs. (A,B) HaCat cells with 70–80% confluence were exposed to 2.5 μM arsenite for 30 min, and the cells were harvested. The PI-3K activity was determined as described in “Materials and Methods.” The results were shown as an autoradiograph (A) and schematic diagram of the PI-3K product PI(3)P from the PI-3K assay spot (CPM) (B). PI-3K activity by arsenite was determined in primary cultured normal human epidermal keratinocytes (C) and schematic diagram of the PI-3K product PI(3)P from the PI-3K assay spot (CPM) (D). The data shown represent one of three independent experiments.