Figure 3.

Requirement of the PI-3K/Akt pathway activation for HaCat cell transformation upon arsenite exposure. (A) HaCat cells stable transfected with dominant negative mutants of Akt (DN-Akt) or p85 (Δp85) or vector control (Mock) were treated with arsenite in different doses as indicated for 180 min. The number was the relative blots density of phosphorylated Akt compared with total Akt. (B) NHEKs were treated with 2.5 μM arsenite at different time points and the phosphorylation of Akt was detected with specific antibodies. (C,D) The anchorage-independent growth was evaluated among the HaCat cells stable transfected with vector control, DN-Akt, and Δp85 after repeated exposure to arsenite for 8 weeks. Each bar indicates the mean and SE of triplicate assay wells.

*Significant decrease compared with that from HaCat cells transfected with vector (Mock) (p < 0.05).