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. 2007 Oct 5;116(1):1–6. doi: 10.1289/ehp.10403

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A key role of cyclin D1 in arsenite-induced HaCat cell transformation. HaCat cells (A) and NHEKs (B) were treated with 5 μM arsenite for the indicated time period (A) or for 24 hr (B), and the cells were extracted with sample lysis buffer for Western blot analysis to determine cyclin D1 expression. (C) HaCat cells stable transfected with vector, DN-Akt, or Δp85, were treated with arsenite at concentrations indicated, and cyclin D1 protein expression levels were evaluated with Western blot analysis. (D) Specific knockdown of cylin D1 in HaCat cells was identified with Western blot analysis compared with normal expression of cyclin D2 expression. (E,F) The capability of anchorage-independent growth activities was compared between cyclin D1 siRNA transfectant and nonspecific control siRNA transfectant after repeatedly treated with arsenite for 8 weeks. Each bar indicates the mean and SE of triplicate assay wells.

*Significant decrease compared with that from HaCat cells transfected with control siRNA (Scramble).