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. 1999 Jun 8;96(12):6787–6790. doi: 10.1073/pnas.96.12.6787

Figure 1.

Figure 1

Lon is stabilized by ATP or AMP-PNP. (A) Stability of the Lon subunit. Lon (2–5 μg) purified and kept in the absence (untreated) or in the presence of 1 mM ATP or 1 mM AMP-PNP was incubated at 30°C for the indicated times and then was analyzed by 10% SDS/PAGE and staining with Coomassie blue. Shown at left are molecular weight standards in kDa. (B) Stability of the Lon oligomer. Lon (5–8 μg) purified and kept in the absence (untreated) or the presence of 1 mM ATP or 1 mM AMP-PNP was incubated at 30°C for the indicated times, was crosslinked with 0.1% glutaraldehyde for 30 min on ice, and was analyzed by 3.3% SDS/PAGE and Coomassie blue staining (34). At left is a recombinant 250-kDa molecular weight marker provided by Amersham Pharmacia.