Table I. Distribution of VSVG within Golgi membranous structures.
Cisternae | Round (>65 nm) vesicular profiles | Buds | |
---|---|---|---|
Nanogold | 1.34 ± 0.12 | 0.16 ± 0.02 | 0.2 ± 0.05 |
Cryogold | 3.8 ± 0.4 | 0.64 ± 0.05 | ND |
Linear density of gold labeling for VSVG expressed as the number of gold particles per intersection between the profiles attributed to each structure (cisternae, vesicles, buds) with the lines of the test grid. Values represent mean ± SEM obtained by analyzing 40 (nanogold) and 20 Golgi stacks (cryogold). Background labeling was low, between 0.01 and 0.015 for both methods.