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. 2001 Dec 24;155(7):1159–1172. doi: 10.1083/jcb.200105093

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Copyright © 2001, The Rockefeller University Press

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Figure 4. — Dynein/dynactin inhibition induces a Mad2-dependent mitotic arrest in PtK₁ cells, but does not block chromosomes congression to the spindle equator or anaphase chromosome segregation and cytokinesis after the checkpoint is inactivated by microinjection of Mad2 antibody. (A) Time-lapse of early prometaphase cell microinjected with p50 (time, 00:00) shows chromosome congression and metaphase arrest. (B) Time-lapse of a prometaphase PtK1 microinjected with p50 (time, 00:02) to induce a mitotic block, and then further injected with Mad2 antibodies to inactivate the checkpoint (00:56), inducing anaphase chromosome segregation and cytokinesis. Time, hrs:min. (C) Graph of anaphase A kinetochore to pole movements for four chromosomes measured (K-P, ♦, ⋄) and anaphase B spindle pole elongation (P-P, ▪) for cell in (B). Not all measured chromosomes are plotted for clarity. Bar, 10 μm.

Figure 4. — Dynein/dynactin inhibition induces a Mad2-dependent mitotic arrest in PtK₁ cells, but does not block chromosomes congression to the spindle equator or anaphase chromosome segregation and cytokinesis after the checkpoint is inactivated by microinjection of Mad2 antibody. (A) Time-lapse of early prometaphase cell microinjected with p50 (time, 00:00) shows chromosome congression and metaphase arrest. (B) Time-lapse of a prometaphase PtK1 microinjected with p50 (time, 00:02) to induce a mitotic block, and then further injected with Mad2 antibodies to inactivate the checkpoint (00:56), inducing anaphase chromosome segregation and cytokinesis. Time, hrs:min. (C) Graph of anaphase A kinetochore to pole movements for four chromosomes measured (K-P, ♦, ⋄) and anaphase B spindle pole elongation (P-P, ▪) for cell in (B). Not all measured chromosomes are plotted for clarity. Bar, 10 μm.