Table II.
Integrated fluorescence of kinetochores from nocodazole cells treated ± ATP inhibitors and immunostained for various spindle checkpoint components and motor proteins
| Kinetochores | Saline | Azide/DOG | Washout |
|---|---|---|---|
| Mad2 | |||
| n | 27 | 28 | 29 |
| mean ± SD | 4.6 ± 1.0 | 4.1 ± 0.8 | 4.4 ± 0.8 |
| ratio to control | 1 | 0.89 | 0.96 |
| BubR1 | |||
| n | 46 | 31 | 36 |
| mean ± SD | 9.2 ± 2.0 | 7.2 ± 2.9 | 9.0 ± 2.2 |
| ratio to control | 1 | 0.78 | 0.98 |
| CENP-E | |||
| n | 30 | 40 | 30 |
| mean ± SD | 21.6 ± 12.1 | 19.5 ± 7.4 | 23.1 ± 3.9 |
| ratio to control | 1 | 0.90 | 1.07 |
| Dynein | |||
| n | 40 | 26 | 24 |
| mean ± SD | 2.4 ± 1.1 | 2.5 ± 0.6 | 2.4 ± 0.5 |
| ratio to control | 1 | 1.04 | 1 |
| 3F3/2 | |||
| n | 46 | 30 | 44 |
| mean ± SD | 15.7 ± 5.1 | 7.4 ± 3.9 | 12.2 ± 3.1 |
| ratio to control | 1 | 0.47 | 0.78 |
Integrated kinetochore fluorescence was measured using identical imaging conditions. With the exception of BubR1 and 3F3/2, all other kinetochore components demonstrated similar mean fluorescence intensities after azide/DOG treatment when compared to saline and washout treatment (P > 0.01). n = number of kinetochores measured per condition. Mean ± SD = actual values/1,000.