Table III. Integrated fluorescence for dynein at kinetochores in nocodazole-treated noninjected and p50-injected cells.
(+) 20 μM nocodazole (30 min) |
||
---|---|---|
Noninjected | p50 injected | |
Dynein | ||
n | 35 | 32 |
mean ± SD | 3824 ± 1611 | 210 ± 559 |
ratio to control | 1 | 0.055 |
Cells were incubated for 30 min in 20 μM nocodazole to enhance visualization of dynein at kinetochores (Hoffman et al., 2001). n = number of kinetochores.