Table II. Relative lateral distribution of Golgi proteins and VSV-G within the Golgi complex.
| Golgi stack (cisternae) | Lateral rims (uncoated) | Coated buds and vesicles | |
|---|---|---|---|
| Man II | 68.9 ± 3.9 | 13.6 ± 2.7 | 17.5 ± 2.8 |
| Giantin | 58.2 ± 2.6 | 18.3 ± 2.8 | 23.5 ± 2.7 |
| KDELr | 35.8 ± 4.3 | 8.9 ± 2.0 | 55.3 ± 4.5 |
| rBet1 | 25.6 ± 4.9 | 15.7 ± 4.0 | 58.7 ± 5.6 |
| VSV-Gts045–GFP (20 min, 32°C) | |||
| anti-GFP | 93.1 ± 1.4 | 4.3 ± 1.3 | 2.6 ± 0.6 |
| anti–VSV-G–lum | 94.1 ± 0.8 | 3.4 ± 0.6 | 2.5 ± 0.5 |
Numbers represent the percentages (mean ± SEM) of the total labeling over the distinct membrane categories and were obtained by analyzing 50 Golgi complexes for each antibody.