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. 2001 Dec 24;155(7):1307–1318. doi: 10.1083/jcb.200102017

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Copyright © 2001, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 5. — GJ assembly between ^S364PCx43, ^S364ECx43 and ^S364ACx43/KO fibroblasts. (A) Example of Lucifer yellow dye transfer between ^S364PCx43 and ^S364ECx43/KO fibroblasts assembled overnight into confluent monolayers (*, injected cell). (B) Representative immunoblot of ^S364PCx43, ^S364ECx43, or ^S364ACx43/KO homogenates after GJ assembly and treatment without (1, 3, 5) or with (2, 4, 6) 8Br-cAMP for the entire assay. To detect ^S364PCx43 and ^S364ECx43 (arrowhead), membranes were labeled with a Cx43 antibody (1:8,000; Sigma-Aldrich) and detected with peroxi- dase-conjugated secondary antibody (1:10,000). A cross-reactive product, which was not consistently detected, is indicated below NP ^S364ECx43 (small arrowhead). (C) Compilation of representative GJ assembly experiments from ^S364PCx43, ^S364ECx43, and ^S364ACx43/KO fibroblasts. Cells were recovered for 4 h, reaggregated for 30 min, and, where indicated, treated with 8Br-cAMP for the entire 4.5 h of the assembly experiment. N values are above the error bars. *0.05 < P < 0.1; **0.01 < P < 0.02.

Figure 5. — GJ assembly between ^S364PCx43, ^S364ECx43 and ^S364ACx43/KO fibroblasts. (A) Example of Lucifer yellow dye transfer between ^S364PCx43 and ^S364ECx43/KO fibroblasts assembled overnight into confluent monolayers (*, injected cell). (B) Representative immunoblot of ^S364PCx43, ^S364ECx43, or ^S364ACx43/KO homogenates after GJ assembly and treatment without (1, 3, 5) or with (2, 4, 6) 8Br-cAMP for the entire assay. To detect ^S364PCx43 and ^S364ECx43 (arrowhead), membranes were labeled with a Cx43 antibody (1:8,000; Sigma-Aldrich) and detected with peroxi- dase-conjugated secondary antibody (1:10,000). A cross-reactive product, which was not consistently detected, is indicated below NP ^S364ECx43 (small arrowhead). (C) Compilation of representative GJ assembly experiments from ^S364PCx43, ^S364ECx43, and ^S364ACx43/KO fibroblasts. Cells were recovered for 4 h, reaggregated for 30 min, and, where indicated, treated with 8Br-cAMP for the entire 4.5 h of the assembly experiment. N values are above the error bars. *0.05 < P < 0.1; **0.01 < P < 0.02.