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. 2003 May 26;161(4):715–725. doi: 10.1083/jcb.200301043

Figure 3.

Figure 3.

Photoadduct formation requires ribosome-bound nascent chains. Translocation intermediates containing a cryptic glycosylation sequence in their nascent chains were incubated without (lanes 1–4) or with (lanes 5–8) puromycin before photolysis. The asterisk identifies photoadducts containing the 60–70-kD protein. Radioactive species are identified as in Fig. 1.