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. 2003 May 26;161(4):715–725. doi: 10.1083/jcb.200301043

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — Immunoprecipitation of the photoadduct containing the 60–70-kD protein. (A) A large sample of photoreactive pPL-sK(QKT) translocation intermediates was prepared as in Fig. 1 (lane 1). Five equal aliquots of this sample were then immunoprecipitated with antisera specific for STT3-A (lane 2), RI (lane 3), RII (lane 4), OST48 (lane 5), or Dad1 (lane 6) before SDS-PAGE. (B) Translation interme- diates with nascent chains of different lengths were photolyzed and immunoprecipitated with STT3-A antiserum. Bands are identified as in the legend to Fig. 1.