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. 2003 May 26;161(4):727–736. doi: 10.1083/jcb.200210156

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — Neuronal but not muscle agrin activates nsk2/musk promoter fragments, depending on an N-box in the first intron. (A) Expression of luciferase activity under the control of the (1.6-N) promoter fragment in adult soleus muscle fibers in vivo is promoted by neuronal but not my muscle Agrin and is blocked upon mutation of the N-box. Muscle fibers were injected intracellularly with the expression plasmids indicated and with pRL-TK for normalization. (B) Agrin-induced expression of luciferase under the control of various nsk2/musk promoter fragments depends on the presence of the N-box in C2C12 myotubes. C2C12 myoblasts were transfected with pRL-TK and the plasmids indicated and grown and differentiated on a laminin substrate with or without mini-Agrin N25₇c21B8 attached. (C) Agrin-induced activation of a 5.4-kb nsk2/musk promoter fragment in C2C12 myotubes is blocked by removal of the intronic sequence containing the N-box. ***P < 0.001 in two-tailed t test. Bars ± SEM.