Figure 10.

Inhibition of DEP-1 enhances phosphorylation of p44/42 MAP kinase and BrdU nuclear incorporation in endothelial cells. (A) Phosphorylation of p44/42 MAP kinase in response to VEGF (80 ng/ml for 10 min) is increased by 80–90% in VEC-positive cells expressing DEP C/S in comparison with control VEC positive. A similar increase is observed after DEP siRNA transfection of the cells. The graph represents the ratio between phosphorylated and total p44/42 MAP kinase as fold increase over untreated VEC-null cells. The values are means ± SD of four independent experiments. Plus and minus indicate the presence or the absence of the protein indicated on the left. (B) Nuclear incorporation of BrdU was stimulated by 80–100% in VEC-positive cells expressing C/S DEP or after DEP siRNA. BrdU incorporation was assayed as in Fig. 1. Data are means ± SD of four experiments.