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. 2003 May 26;161(4):793–804. doi: 10.1083/jcb.200209019

Figure 3.

Figure 3.

VE-cadherin expression and clustering reduce the extent of p44/42 MAP kinase phosphorylation in response to VEGF. (A) Confluent and sparse VEC-null and VEC-positive endothelial cells were stimulated with VEGF (80 ng/ml for 10 min), and phospho p44/42 MAP kinase and total MAP kinase were assayed by Western blot with specific antibodies. The columns represent the ratio between phosphorylated and total values as fold increase over the ratio calculated in sparse unstimulated VEC null. VEGF-stimulated phosphorylation of MAP kinases was reduced at confluence only in VEC-positive cells. The mean ± SD of three independent experiments is reported. In a total of 12 experiments, the increase of p44/42 MAP kinase phosphorylation in VEC-null cells ranged from three- to sixfold over VEC-positive cells. (B) In confluent HUVEC, phosphorylation of p44/42 MAP kinase in response to VEGF (80 ng/ml for 10 min) was reduced about threefold in comparison with sparse cells. Column values are as in A.