Figure 8.

Expression of tyrosine phosphatase DEP-1 in VEC-positive and -negative cells and effect of transfection of wild-type and DEP-1 C/S constructs and DEP-1 RNAi. In VEC-positive endothelial cells (third lane), DEP-1 was 80–90% higher than in VEC null (fourth lane). In VEC-positive cells, transfection of wild-type (wt, first lane) or point-mutated (C/S, second lane) DEP-1 constructs resulted in increased expression of the protein (50–60% more than in control VEC positive). siRNA directed against DEP-1 (DEP siRNA) resulted in 35–40% inhibition of DEP expression (fifth lane). Scramble oligonucleotides (scr siRNA), used as control (100 nM as DEP siRNA oligonucleotide), were ineffective. Plus and minus indicate the presence or the absence of the protein indicated on the left. The columns report the mean of four experiments ± SD.