Table.
AII. Additional parameters for model of PH-GFP translocation
| Parameter | Value | Comments |
|---|---|---|
| KdPIP2PH | 2.0 μM | Hirose et al., 1999 |
| KdIP3PH | 0.1 μM | Hirose et al., 1999 |
| PH_GFP_Cyt_init | 2.31 μM | Calculated from measured total PH-GFP of 6 μM, basal InsP3 of 0.16 μM, and literature value of KdIP3PH of 0.1 μM |
| IP3_PHGFP_Cyt_init | 3.70 μM | Calculated from measured total PH-GFP of 6 μM, basal InsP3 of 0.16 μM, and literature value of KdIP3PH of 0.1 μM |
| PHGFP_Diff | 50 μm2/s | Diffusion coefficient estimated from molecular weight |
| IP3_PHGFP_Diff | 50 μm2/s | Diffusion coefficient estimated from molecular weight |
| PIP2_PHGFP_PM_init | 4,617.4 molecules/μm2 | Calculated from measured total PH_GFP_Cyt_init of 2.30769 μM, basal PIP2 of 4,000 molecules/μm2, and literature value of KdPIP2PH of 2 μM (Hirose et al., 1999) |
| kf_IP3PH | 10.0 (μM·s)−1 | Near diffusion controlled |
| kf_PIP2PH | 0.12 (μM·s)−1 | van der Wal et al., 2001 |