Table 3.
Effects of S-diclofenac and diclofenac on reduced GSH formation in paced isovolumic left rabbit heart preparations subjected to low-flow ischaemia and reperfusion
| Treatment |
GSH (nmol min−1 per g w.t.) |
||
|---|---|---|---|
| Pre-ischaemia | Reperfusiona | ||
| Vehicle | 0.53±0.06 | 5.18±0.67 | |
| S-diclofenac | 3 μM | 0.49±0.06 (−8) | 5.53±0.44 (+7) |
| S-diclofenac | 10 μM | 0.66±0.11 (+24) | 7.15±0.62* (+38) |
| S-diclofenac | 30 μM | 0.81±0.09* (+52) | 8.29±0.49** (+60) |
| Diclofenac | 3 μM | 0.57±0.10 (+7) | 5.07±0.30 (−2) |
| Diclofenac | 10 μM | 0.52±0.06 (−2) | 4.61±0.56 (−11) |
| Diclofenac | 30 μM | 0.46±0.05 (−13) | 3.98±0.47 (−23) |
Abbreviations: GSH, glutathione; S-diclofenac, 2-[(2,6-dichlorophenyl)amino]benzeneacetic acid 4-(3H-1,2-dithiole-3-thione-5-yl)-phenyl ester; w.t., wet tissue.
Data are mean±s.e.mean of seven heart preparations per group. Values in the parentheses show, percentage from vehicle-treated hearts. Drugs were infused for 20 min before flow rate reduction.
a
Data refer to the first 10 min of reperfusion.
*P<0.05 and **P<0.01 versus vehicle-treated hearts.