Figure 2.

Effects of Ang II, Ang IV, pACE2 gene transfer and angiotensin antagonists on mRNA for p22phox in endothelial cells. The mRNA was analysed by real-time PCR and is expressed as log copy ratio of p22phox/GAPDH. The stimulatory effects of Ang II or Ang IV on the mRNA for p22phox expression was prevented by pACE2. The AT₁ selective antagonist losartan, but not the AT₂ antagonist PD123369, also prevented the stimulation by Ang II (a) whereas that of Ang IV was prevented by the selective AT₄ antagonist, divalinal-Ang IV. (b) Data shown are mean±s.d. (n=4–6). ^**P<0.01 vs control; ^##P<0.01 vs Ang IV alone. ACE2, angiotensin-converting enzyme 2; Ang II, angiotensin II; Ang IV, angiotensin IV;AT₁, angiotensin type 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.