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. 2007 Oct 1;153(1):66–74. doi: 10.1038/sj.bjp.0707482

Figure 4.

Figure 4

Effects of pACE2 gene transfer on MIF mRNA (normalized to human GAPDH) in endothelial cells, stimulated with Ang II or Ang IV. Regular RT-PCR (a, n=4), real-time PCR analysis (b and c, n=5–6) disclosed the stimulatory effects of Ang II or Ang IV on the mRNA expression of MIF. In cells pretreated with pACE2 or the antagonists, losartan or divalinal-Ang IV, this stimulation was prevented. Values are mean±s.d. **P<0.01 vs control; #P<0.05 ##P<0.01 vs Ang II or Ang IV alone. ACE2, angiotensin-converting enzyme 2; Ang II, angiotensin II; Ang IV, angiotensin IV; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MIF, macrophage migration inhibitory factor; RT, reverse transcription.