Table 1.
Experimental condition | Control | Precond | EPO 25 | EPO 50 | EPO 150 | EPO+LY | LY | EPO+ U0 | U0 | Diab Control | Diab+EPO |
---|---|---|---|---|---|---|---|---|---|---|---|
Weight (mg) | 0.46 | 0.41 | 0.61 | 0.56 | 0.57 | 0.88 | 0.89 | 0.51 | 0.52 | 0.55 | 0.77 |
s.e. | 0.10 | 0.06 | 0.09 | 0.11 | 0.10 | 0.33 | 0.15 | 0.06 | 0.05 | 0.12 | 0.08 |
Diameter (mm) | 0.90 | 0.78 | 0.82 | 0.98 | 0.92 | 1.00 | 0.96 | 0.93 | 0.85 | 0.76 | 0.9 |
s.e. | 0.06 | 0.08 | 0.06 | 0.06 | 0.03 | 0.05 | 0.07 | 0.06 | 0.07 | 0.09 | 0.06 |
Length (mm) | 3.80 | 3.70 | 5.30 | 3.80 | 4.80 | 6.60 | 6.10 | 5.64 | 5.53 | 5.73 | 5.10 |
s.e. | 0.35 | 0.15 | 0.31 | 0.22 | 0.70 | 0.74 | 0.35 | 0.49 | 0.85 | 0.63 | 0.64 |
Cross section (mm2) | 0.71 | 0.61 | 0.56 | 0.78 | 0.50 | 0.82 | 0.76 | 0.69 | 0.59 | 0.49 | 0.72 |
s.e. | 0.09 | 0.08 | 0.08 | 0.09 | 0.46 | 0.09 | 0.10 | 0.09 | 0.08 | 0.12 | 0.09 |
Sample number | 8 | 7 | 9 | 10 | 6 | 7 | 8 | 8 | 6 | 7 | 8 |
The values are represented as means with s.e., from the number of samples given at the bottom of each column. All samples were from non-diabetic patients, except for the last two columns. Experimental conditions were as follows: All trabeculae were subjected to hypoxia-reoxygenation and randomized to the following groups: Control: untreated trabeculae; Precond: with preconditioning hypoxia; EPO 25, 50, 150: treated with EPO 25, 50 or 150 ng ml−1; EPO+LY: EPO 50 ng ml−1+ the PI3K inhibitor LY294002; LY: LY294002 alone; EPO+UO: EPO 50 ng ml−1+ the ERK 1/2 inhibitor U0126; UO: U0126 alone; Diab control: untreated trabeculae from diabetic patients; Diab+EPO: diabetic trabeculae+EPO 50 ng ml−1.