Skip to main content
. 1999 Jun 8;96(12):6791–6796. doi: 10.1073/pnas.96.12.6791

Figure 3.

Figure 3

Quantitation of the amount of HA-HA SMAD2 protein in animal cap cells. (A) Western blotting of HA-HA SMAD2. Each embryo was injected with the indicated dose of HA-HA Smad2 mRNA. Animal caps were homogenized at stage 10.5. Twelve micrograms of total protein from mRNA-injected embryos (lanes 1–3) or the indicated amount of recombinant HA-HA SMAD2 protein (lanes 4–6) was subjected to SDS/PAGE, followed by Western blotting with the anti-HA antibody. The result shown is representative of three independent experiments. (B) Quantitation of the amount of HA-HA SMAD2 protein. The amounts of HA-HA SMAD2 protein in lanes 2 and 3 of A were quantified by using a standard curve based on recombinant HA-HA SMAD2 protein. (C) Quantitation of the amount of HA-HA SMAD2 protein synthesized by embryos injected with various doses of HA-HA Smad2 mRNA. Each embryo was injected with the indicated dose of HA-HA Smad2 mRNA. The amount of HA-HA SMAD2 protein per 12 μg of total protein was quantified as described in B. The results shown are drawn from two independent experiments. In a third experiment, all points were somewhat higher, but fell on a straight line.