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GTPase Activity of Wild-Type and Mutant Dynamin
| Dynamin | Rat brain | Wild-type | Dynamin T65A |
|---|---|---|---|
| μM | |||
| 0.5 | 3.5 ± 0.6 | 3.8 ± 0.3 | 0.25 ± 0.01 |
| 1.5 | 5.5 ± 0.7 | 8.0 ± 1.0 | 0.05 ± 0.01 |
GTPase assays were carried out as described by Warnock et al. 1996. Results are expressed as the mean ± SEM of four different determinations of the specific activity (pmol GTP hydrolyzed/min/pmol dynamin) of dynamin GTPase.
