Figure 4.

Live cell imaging of spc24-1 cells containing GFP-Spc42p, an SPB component, and GFP-CEN5. CEN5 was labeled by integration of tet operators (which bind GFP-tet repressors) 1.4 kb from CEN5 (Tanaka et al. 2000). Cells were synchronized in G₁ with α-factor and released at 23°C for 1.5 h (a) or at 36°C (b1–b3, c1–c2) for the times indicated; arrowheads show the SPBs which are brighter than the centromeres (He et al. 2000; Tanaka et al. 2000). The same cell is imaged in b1–b3 and another cell in c1–c2. Bar, 2 μm.