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. 2001 Jan 22;152(2):349–360. doi: 10.1083/jcb.152.2.349

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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(a) Live cell imaging of SpNdc80p-GFP (b1 and b2), SpNuf2p-GFP (c1 and c2), and SpSpc24p-GFP (d1 and d2) in S. pombe. Cells about to complete anaphase A (b1, c1, and d1) show between five and six spots which within a few minutes coalesce into two spots during anaphase B (b2, c2, and d2). (e–g) Fluorescence of GFP compared with immunofluorescence with anti-Sad1 (e), antitubulin (f), and anti–HA-tagged Bub1 (g). Bars, 2.5 μm.