Table 2.

Nontelomeric Late Origins Do Not Colocalize with Telomeres

Probe 1(Dig)	Probe 2(Alexa-546)	Colocalization	n (signal pairs, nuclei)
ARS1412	ARS1413	70%	33, 27
p12	ARS1413	16%	49, 36
ARS1412	Y'	32%	41, 32
Y′	ARS1413	33%	71, 56
Y′	ChrIV-257	30%	27, 20
Y′	ARS603	27%	30, 23
HML	Y'	68%	69, 60
CEN8	Y'	27%	60, 42
Y′-tel	Y'-tel	88%	34, 23

Colocalization of FISH signals representing subtelomeric regions (Y′), the indicated late-firing ARS elements, the centromeric region from chromosome VIII (CEN8), and a region flanking the HML locus on the left arm of chromosome III: data are taken from experiments shown in Fig. 4c and Fig. d, and Fig. 5, a–d, and from additional experiments in which the fluorescent markers were switched such that the same probe was not always labeled with Dig-dUTP (see column labeled Probe 1/Dig) or with Alexa-546-dUTP (Probe 2/Alexa-546). For quantifying signals, midcell focal planes were examined for the presence of at least one signal of each labeled probe. Cells with more than two of each were omitted from evaluation, unless the more abundant signal was the subtelomeric Y′ probe. Cells displaying two signals from each probe were scored as two signal pairs. Overlap of signal areas >25% above a given threshold was defined as colocalization (see Materials and Methods).