Table 1.
Filter disk assay for mercury sensitivity in Escherichia coli strain SK1592 with plasmid pDU202-merA+
| Metal salt | Concentration, M | Zone diameter, mm
|
|
|---|---|---|---|
| pBSSKI1-merB− | pmerBpe-merB+ | ||
| HgCl2 | 0.1 | 18 | 20 |
| PMA | 0.1 | 22 | 8 |
| MeHgCl | 0.1 | 26 | 10 |
E. coli strain SK1592 containing the various mer gene and control plasmids were assayed for mercury and organomercurial resistance. Each 6-mm filter diameter disk contained 2 μl of freshly prepared metal salt stock solution. The genotype of each strain and sensitivity (s) or resistance (r) phenotype on different forms of mercury is indicated below the strain and plasmid designations.