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Spindle reformation after nocodazole treatment. Oocytes were cultured in the presence of 10 μg/ml nocodazole from 3 to 5 h (A–D) and from 6 to 8 h (E–H) after GVBD. Microtubules appear in green and chromosomes in red. Oocytes were fixed 20 (A and E), 50 (B and F), 120 (C and G), and 150 min (D and H) after removal of the drug.
