Table 2.
Effects of Various Incubations in Nocodazole (10 μg/ml) on the Timing of Polar Body Extrusion
| Incubation in nocodazole | n | Time of PBE | Delay | Time required for PBE after nocodazole removal | Δ |
|---|---|---|---|---|---|
| h after GVBD | min after GVBD | min | min | ||
| Control | 92 | 483 ± 29 | — | — | |
| 2 to 4 | 75 | 495 ± 33 | 12 | 255 | |
| 2 to 5 | 78 | 503 ± 26 | 20 | 203 | |
| 4 to 5 (1 h incubation) | 73 | 505 ± 35 | 22 | 205 | 2 |
| 2 to 6 | 70 | 538 ± 29 | 50 | 178 | |
| 5 to 6 (1 h incubation) | 74 | 532 ± 34 | 49 | 172 | 6 |
| 2 to 7 | 88 | 584 ± 29 | 101 | 164 | |
| 6 to 7 (1 h incubation) | 57 | 577 ± 27 | 94 | 157 | 7 |
| 2 to 8 | 88 | 643 ± 21 | 160 | 163 | |
| 7 to 8 (1 h incubation) | 57 | 634 ± 36 | 151 | 154 | 9 |