Figure 5.

The effects of calpain inhibitor I and CPT-cAMP on cell migration. Cells were grown to confluence and quiesced for 48 h in DME with 0.1% dialyzed FBS before treatment with or without IP-10 (1–50 ng/ml), EGF (1–10 nM), calpain inhibitor I (1 ng/ml), and/or CPT-cAMP (10 μM). Cell migration assays were performed as described in Materials and Methods. The data are shown as the ratio to the 1 nM EGF-induced cell migrative activity. The data are the mean ± SEM of at least three independent studies each performed in triplicate. Statistical analyses were performed by Student's t test.