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. 1999 Jul 12;146(1):243–254. doi: 10.1083/jcb.146.1.243

Figure 6.

Figure 6

EGF-induced calpain activation. (a) Cells were grown to confluence in 10-cm tissue culture plates and quiesced for 48 h with DME containing 0.1% dialyzed FBS. Cells were treated with IP-10 (50 ng/ml) for 4 h or CPT-cAMP (20 μM) for 30 min before EGF (1 nM) for 30 min. Calpain activities were analyzed by measuring the elevation in fluorescence intensity that occurs upon calpainolytic digestion of dichlorotriazinylamino-fluorescein–labeled MAP2. The data are shown as a ratio to EGF-induced calpain activation. The data are the mean ± SEM of at least three independent studies. Statistical analyses were performed by Student's t test. (b) Cells were grown to confluence in 6-well tissue culture plates and quiesced for 48 h. Cells were treated with IP-10 (50 ng/ml) for 4 h before EGF (1 nM) for 30 min. Cell lysates were separated by SDS-PAGE, transferred to Immobilon-P (Millipore), and immunoblotted with an anti–calpain I or –calpain II antibody (Biomol).