Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Jul 12;146(1):243–254. doi: 10.1083/jcb.146.1.243

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

graphic file with name JCB9812049.f8a.jpg — The effects of ELR-negative CXC chemokines on EGF-induced cell migration (a), EGF-induced calpain activation (b), and PDGF-induced motility (c). (a) Cells were grown to confluence and quiesced for 48 h before treatment with or with out IP-10 (50 ng/ml), MIG (100 ng/ml), PF4 (50 ng/ml), and/or EGF (1 nM). Cell migration assays were performed as described in Materials and Methods. The data are shown as the ratio to the 1 nM EGF-induced cell migrative activity. The data are the mean ± SEM of at least three independent studies each performed in triplicate. Statistical analyses were performed by Student's t test as compared with EGF-induced cell migrative capacity in the absence of CXC chemokines: **P < 0.01. (b) Cells were grown to confluence in 10-cm tissue culture plates and quiesced for 48 h. Cells were treated with or without IP-10 (50 ng/ml), MIG (100 ng/ml), and/or PF4 (50 ng/ml) for 4 h before EGF (1 nM) for 30 min. Calpain activities were analyzed by measuring the elevation in fluorescence intensity that occurs upon calpainolytic digestion of dichlorotriazinylamino-fluorescein–labeled MAP2. The data are shown as a ratio to EGF-induced calpain activation. The data are the mean ± SEM of at least three independent studies. Statistical analyses were performed by Student's t test as compared with EGF-induced calpain activation in the absence of CXC chemokines: **P < 0.01. (c) Cells were grown to confluence and quiesced for 48 h before treatment with or without IP-10 (50 ng/ml), Rp-8-Br-cAMPS (50 μM), and/or PDGF-BB (5 ng/ml). Cell migration assays were performed as described in Materials and Methods. The data are shown as the ratio to the 5 ng/ml PDGF-induced cell migrative activity. The data are the mean ± SEM of two independent studies each performed in triplicate. Statistical analyses were performed by Student's t test.

graphic file with name JCB9812049.f8b.jpg — The effects of ELR-negative CXC chemokines on EGF-induced cell migration (a), EGF-induced calpain activation (b), and PDGF-induced motility (c). (a) Cells were grown to confluence and quiesced for 48 h before treatment with or with out IP-10 (50 ng/ml), MIG (100 ng/ml), PF4 (50 ng/ml), and/or EGF (1 nM). Cell migration assays were performed as described in Materials and Methods. The data are shown as the ratio to the 1 nM EGF-induced cell migrative activity. The data are the mean ± SEM of at least three independent studies each performed in triplicate. Statistical analyses were performed by Student's t test as compared with EGF-induced cell migrative capacity in the absence of CXC chemokines: **P < 0.01. (b) Cells were grown to confluence in 10-cm tissue culture plates and quiesced for 48 h. Cells were treated with or without IP-10 (50 ng/ml), MIG (100 ng/ml), and/or PF4 (50 ng/ml) for 4 h before EGF (1 nM) for 30 min. Calpain activities were analyzed by measuring the elevation in fluorescence intensity that occurs upon calpainolytic digestion of dichlorotriazinylamino-fluorescein–labeled MAP2. The data are shown as a ratio to EGF-induced calpain activation. The data are the mean ± SEM of at least three independent studies. Statistical analyses were performed by Student's t test as compared with EGF-induced calpain activation in the absence of CXC chemokines: **P < 0.01. (c) Cells were grown to confluence and quiesced for 48 h before treatment with or without IP-10 (50 ng/ml), Rp-8-Br-cAMPS (50 μM), and/or PDGF-BB (5 ng/ml). Cell migration assays were performed as described in Materials and Methods. The data are shown as the ratio to the 5 ng/ml PDGF-induced cell migrative activity. The data are the mean ± SEM of two independent studies each performed in triplicate. Statistical analyses were performed by Student's t test.