Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Jul 12;146(1):13–28. doi: 10.1083/jcb.146.1.13

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Specificity of affinity-purified anti-Drosophila Bub1 antibodies. Identical amounts of Drosophila third instar larval brain extracts, from either wild-type (Oregon R) or bub1 mutant homozygotes, were loaded onto the indicated lanes of a Western blot probed with affinity-purified anti-Drosophila Bub1 antibodies (see Materials and Methods). A doublet of ∼165 kD (the predicted size for Drosophila Bub1) is recognized in wild-type extracts. These two bands are almost completely absent in brain extracts made from bub1 mutant brains. The antibody also recognizes a band of ∼100 kD that is not Bub1-specific and that is also recognized by pre-immune IgY. This cross-reacting band serves as an internal loading control.