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. 1999 Jul 12;146(1):13–28. doi: 10.1083/jcb.146.1.13

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Drosophila Bub1 is recruited to kinetochores. DNA is shown in blue and Bub1 is in red. (A) A chromosome isolated from a Drosophila S2 cell arrested with colchicine, showing strong Bub1 staining at the kinetochores. (B) Bub1 is localized to the kinetochores in a wild-type (Oregon-R) neuroblast arrested with colchicine. (C) A neuroblast from a bub1 mutant (l(2)K06109/l(2)K06109) brain arrested with colchicine and stained with affinity-purified anti-Bub1 antibodies under identical conditions to those used in B. Note the complete absence of Bub1 staining in this figure; complete lack of Bub1 staining is also observed in all other bub1 allelic and deficiency combinations (not shown). D–F show that Bub1 (red) colocalizes with the kinetochore marker ZW10 (green) in wild-type neuroblasts, although the levels of staining of individual kinetochores with the two reagents are not always in concert. Bars, 5 μm. A–C are at the same magnification, as are D–F.