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. 1999 Jul 12;146(1):125–140. doi: 10.1083/jcb.146.1.125

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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SRO7 and SRO77 form a functionally redundant gene family and the sro7Δ, sro77Δ mutant exhibits a severe cold-sensitive phenotype. (A) Tetrad dissection of a cross between sro7Δ and sro77Δ single disruptants. Tetrads were dissected and grown on YPD plates at 25°C. Pinpoint colonies correspond to haploid segregants containing disruptions of both SRO7 and SRO77. Large colonies correspond to haploid segregants containing wild-type copies of both SRO7 and SRO77 or individual disruptions in either gene. T refers to a tetratype and NPD refers to nonparental ditype segregation patterns observed after scoring for auxotrophic markers for each disruption. (B) Cold sensitivity of the sro7Δ, sro77Δ double-disruptant strain. Each strain of a tetratype tetrad was struck out onto YPD plates and grown at either 37, 25, or 14°C. The genotype of the four strains is as indicated.