Table 1.
Summary of Suppression Properties of High Copy SR07 and SEC9
| Mutant | 2μ SRO7 | 2μ SEC9 |
|---|---|---|
| rho3Δ | ++ | +++ |
| sro7Δ, sro77Δ | +++ | − |
| sec4-P48 | + | +++ |
| sec1-1 | − | ++ |
| sec2-41 | − | − |
| sec3-2 | ++ | ++ |
| sec4-8 | − | − |
| sec5-24 | − | − |
| sec6-4 | − | − |
| sec8-9 | ++ | ++ |
| sec9-4 | − | +++ |
| sec10-2 | + | − |
| sec15-1 | +++ | ++ |
Late acting sec and rho3 mutants were transformed with either vector (pRS426), SRO7 (pB497), or SEC9 (pB35). Transformants grown on selective media were picked into microtiter plates and tested on YPD plates for growth at four temperatures: 25°, 30°, 34°, and 37°C. Scoring for temperature-sensitive mutants is as follows: triple plus, wild-type growth at the most restrictive temperature (37°C); double plus, good growth at an intermediate temperature (34°C) at which empty vector controls cannot grow; and single plus, slow growth at an intermediate temperature. Scoring for suppression of cold-sensitive mutants was determined by comparing growth relative at 14°C to an isogenic wild-type yeast (+++) and to the vector control (−).