Table 2.
Female fertility and development time of S. oryzae progeny from crosses between naturally symbiotic Chinese strain (Ch), or its SOPE-aposymbiotic heat-treated relative (ChH), and their tetracycline-treated derivatives (ChT and ChHT)
| Parents
|
Progeny
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Cross
|
Female
|
Male
|
Mean fertility, 14-d progeny | nb1 | Mean development time, days | nb2 | |||
| No. | F × M | W | S | W | S | ||||
| 1 | Ch × Ch | + | + | + | + | 57.60 ± 6.37 | 10 | 34.81 ± 0.27 | 159 |
| 2 | Ch × ChT | + | + | − | − | 51.71 ± 4.35 | 7 | 33.99 ± 0.35 | 46 |
| 3 | ChT × Ch | − | − | + | + | 4.80 ± 2.29* | 5 | 48.00∗ | 1 |
| 4 | ChT × ChT | − | − | − | − | 18.40 ± 4.37* | 5 | 35.66 ± 0.26 | 92 |
| 5 | ChH × ChH | + | − | + | − | 28.85 ± 1.05 | 20 | 38.17 ± 0.43 | 78 |
| 6 | ChH × ChHT | + | − | − | − | 25.51 ± 1.47 | 21 | 41.25 ± 0.49∗ | 51 |
| 7 | ChHT × ChH | − | − | + | − | 6.84 ± 1.15* | 17 | 40.04 ± 0.76∗ | 26 |
| 8 | ChHT × ChHT | − | − | − | − | 21.15 ± 2.32* | 20 | 44.41 ± 0.30∗ | 138 |
Wolbachia (W) and SOPE (S) were detected by FISH and PCR using respective specific 16S probes and primers. 14-d progeny, mean progeny per female from a 14-day egg-laying of 2- to 3-week-old females (results are mean ± SE); nb1, pair number; nb2, progeny number from a 2-day egg-laying; +, presence; −, absence. ∗, Significant differences compared with the control crosses of each group—i.e., 1 for 2, 3, 4, and 5 for 6, 7, 8 (bilateral Dunnett test, α = 0.05).