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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Immunity. 2007 Aug 16;27(2):203–213. doi: 10.1016/j.immuni.2007.07.007

Figure 1. Primary immune responses of naive CD4+ T cells vary with respect to pMHC specificity.

Figure 1

A, Flow cytometry gates used in all figures to identify T cells from total spleen and lymph node cells: side scatter-widthlow (left plot); CD3+, non-T cell lineage- (middle plot); and either CD4+ or CD8+ (right plot). B, Where indicated, B6 mice were injected i.v. with 50 µg of the indicated peptide plus 5 µg LPS. Eight days later, spleen and lymph node cells were harvested and stained with the indicated tetramer. Representative contour plots of CD44 versus the indicated tetramers are shown for non-T cell lineage-, CD3+, CD4+ events gated from 106 total collected events. The total number of cells for each individual mouse shown is indicated below the relevant gate. Mean values ± S.D. were 2W1S:115,000 ± 40,000; FliC: 23,000 ± 20,000; OVA: 7,000 ± 3,000 (n=4 per group).