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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Immunity. 2007 Aug 16;27(2):203–213. doi: 10.1016/j.immuni.2007.07.007

Figure 6. Naive CD4+ T cell population diversity is related to population size.

Figure 6

A, Total lymph node and spleen cells from naive B6 mice (top) or B6 mice injected i.v. with 50 µg 2W1S peptide plus 5 µg LPS 4 days earlier (bottom) were stained with antibodies to TCR Vβ4 and Vα2. 2W1S:I-Ab- and 2W1S:I-Ab+ gated events are represented in histograms by shaded profiles and solid lines, respectively. B, TCR Vβ and Vα gene segment usage in total CD4+ T cells from naive mice (unfilled bars), or 2W1S:I-Ab+ CD4+ T cells from naive mice (filled bars) or mice injected i.v. with 50 µg 2W1S peptide plus 5 µg LPS 4 days earlier (hatched bars). Data are mean values ± S.D. from 4∓8 mice per group. C, Usage profile for TCR Vβ gene segments in total CD4+ T cells from naive B6 mice or tetramer+ CD4+ T cells from B6 mice injected i.v. with 50 µg of the indicated peptide plus 5 µg LPS 8 days earlier. Data are values from pooled samples taken from 4–10 mice per group, or D, samples taken from 4 individual mice per group.