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The murine Xist gene consists of seven exons. (A) Revised genomic structure of Xist contains a 781-bp-long intron compared with the documented structure of exon VI by Brockdorff et al. (14). Exon VI/exon VII junction sequences are shown. Arrowhead, AvaI; asterisk, EcoRI. (B) Comparison of AvaI/EcoRI fragments from mouse genomic DNA, Y116 (lane 2), and female mouse lung cDNA (lane 3). PCR fragment spanning the region was cloned and sequenced. Lane 1 is 1-kb DNA size marker (GIBCO/BRL).
