Figure 3.

Northern blot showing major and minor Xist species. Murine male (♀) and female (♂) kidney RNA was fractionated on a formaldehyde-agarose gel and transferred to positively charged nylon yielding duplicate strips. Duplicate lanes were hybridized to the individual probes: pWS850, pWS854, and mx8 (20). The lane containing mx8 is not shown, because there was no hybridization signal. Xist transcripts, both major and minor, are indicated by arrow symbols. Positions of ribosomal RNA are indicated to give an indication of relative mobility. The figure shows two major species of Xist using the pWS850 probe. The new 3′-end probe, pWS854, hybridizes disproportionately to the larger of the two major species of Xist RNA.