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. 1999 Jun 8;96(12):6835–6840. doi: 10.1073/pnas.96.12.6835

Figure 1.

Figure 1

Events of meiosis in WT and gcn5–21 mutant cells. (A) Meiotic DNA replication in WT (NKY3221) and gcn5–21 mutant cells (NKY3222) at the indicated times points after transfer to SPM. (B) Meiotic divisions (MI ± MII) in the same time course as A. (C) HIS+ prototroph formation in GCN5/gcn5–21 cells (NKY3203) and gcn5–21/gcn5–21 mutant cells (NKY3202) returned to vegetative growth at the indicated times after transfer into SPM. Cells were plated onto synthetic complete media (SC) and onto media lacking histidine (SC-HIS). (D) Meiotic DNA double-strand breaks near the THR4 locus in gcn5–21 (NKY3198) and WT (NKY895) cells at the indicated time points after transfer to SPM assayed by Southern blot analysis.