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. 1999 Jun 8;96(12):6835–6840. doi: 10.1073/pnas.96.12.6835

Figure 3.

Figure 3

Acetylation of histone H3 and histone H4 in total DNA and at the IME1 and IME2 promoters under vegetative and meiotic conditions. (A) Schematic of probes used. (B) Results of chromatin IP using antibodies to acetylated histone H3 or H4. WT cells (NKY3222) sporulated at 88% efficiency and gcn5–21 (NKY3221) and rpd3Δ (NKY3227) mutants sporulated at <1% efficiency after 24 hr in SPM. (C) Relative IP levels are shown after normalization to YPD-WT levels. (D) The ratio of precipitated IME2 fragment to precipitated genomic DNA from the experiment shown in B (Left) and from three independent experiments (± SD; Right).