Table 1.

Sporulation efficiency on nonfermentable carbon sources

Strain	Relevant genotype	Acetate	Ethanol	Glycerol	Acetate + glycerol	No carbon	Spore viability*
NKY3222	GCN5 RPD3	88%	49%	70%	55%	71%	96%
NKY3221	gcn5 RPD3	<1%	<1%	26%	3%	25%	94%
NKY3226	GCN5 rpd3	<1%	2%	39%	4%	24%	90%
NKY3227	gcn5 rpd3	<1%	<1%	<1%	<1%	<1%	No sporulation

Cells were grown on solid YPD media for 2 days before patching on sporulation plates. All media contain 0.2 M Mes, pH 5.5, 0.1× amino acid drop out powder, 2% agar plus 0.3% of each carbon source. Sporulation was assayed after 3 days. 

^*Twelve four-spore asci were dissected from glycerol-containing media onto YPD plates to measure spore viability.