Table 3.

Locations and directions of transposon insertions.

genea	strain number	9 bp direct repeatb	last nucleotide transcribed
clpP	I35	ACTTTGCAC	34
	I75	ACATGGCTA	163
crp	I42	TCACATTAC	−114c
	I100	GTGCTTGGC	12
	I104	GGGTAAAGA	176
	I105	CGGTAAAGA	608
cyaA	I65	GTGTGGAAG	451
	I102	GCATATACT	542
	I103	CCGCAGGAT	1242
hns	I28	AACCTGGAC	329
icdA	I37	GTTTGAAGC	1010
	I107	CTCCAAGCC	418
intQ	I6	GGGTTAAGC	2
mcrA/elbA	I24	GTTCAGAGT	+361d
	I44	GGGTTGGAC	+248d
moaD	I19	ACCATCCGC	196
mobA	I22	GCCTTACAT	314
recA	I7	GTCCAACAC	563
	I78	GGTGAAGAC	99
recB	I40	GGTATGTGC	1568e
	I57	GTTCGATGG	1940
	I68	GGATGTGGT	1922
	I73	GATTTACAC	3470
	I79	GGTATGTGC	1568e
	I83	TTCTTGCAC	2868
	I93	ACCAGGCCT	491
	I94	ATGGTGAAC	1365
recC	I32	GATAGCAGT	1119
	I36	CCCGCCACC	1558
	I50	GCTGGGAAT	2039
	I52	GTCTGGACG	34
	I54	ACATGATGG	3241
	I85	GGGTAAACA	699
	I90	GCCTGGCAC	442
	I97	GTTGTTGGG	1601
trkH	I23	TAACTACGG	761
	I27	GATTATCCC	71
tufA	I29	CACGTAGAC	959
tufB	I17	GGCGTAGAG	1047

^aAlternate gene names: elbA (ycgW), intQ (b1579)

^bThe 9 bp repeat was determined using a single primer from within the transposon. The last base of the inferred repeat shows the last intact nucleotide of the gene, reading in the 5′ to 3′ direction with respect to transcription.

^cThis insertion is located 115 nucleotides upstream of the start codon of the crp gene.

^dThese insertions lay 361 and 248 nucleotides downstream of the stop codon of the mcrA gene.

^eThese insertions, while identical, were isolated independently and are therefore not clones.